N. Botsoglou et al., Rapid gas chromatographic method for simultaneous determination of cholesterol and alpha-tocopherol in eggs, J AOAC INT, 81(6), 1998, pp. 1177-1183
A new method was developed for simultaneous determination of cholesterol an
d alpha-tocopherol in eggs. It involves rapid and simple sample preparation
accomplished in one tube and chromatographic separation that does not requ
ire derivatization of analytes. Total analysis time per sample is 40 min. L
abor, cost, and use of hazardous chemicals are minimized. To ensure selecti
vity, accuracy, and precision, critical analytical parameters were investig
ated. Overall recoveries were 98.8 and 99.2% for cholesterol and alpha-toco
pherol, respectively. Linearity was acceptable for both analytes (r = 0.996
4 for cholesterol and 0.9996 for alpha-tocopherol) in the fortification ran
ge examined. Precision data based on within-day and between-days variation
gave overall relative standard deviations of 2.0% for cholesterol and 7.0%
for alpha-tocopherol. The method was applied successfully for quantitation
of cholesterol and alpha-tocopherol in eggs.