Determination of adenosine and deoxyadenosine in urine by high-performanceliquid chromatography with column switching

The means of measurement of adenosine and deoxyadenosine in urine was devel oped by separating adenosine and deoxyadenosine from other compounds using high-performance Liquid chromatography with column switchings. This method is simple and convenient since no pretreatment of the urine is needed. Usin g this method, it could be demonstrated that urinary adenosine was higher i n an adenosine deaminase (ADA) deficient patient who had a bone marrow tran splant treatment (1.97 mu mol/mmol creatinine) and in a heterozygote who ha d a markedly low erythrocyte ADA activity (1% of control ADA activity) (1.3 3 mu mol/mmol creatinine) as compared to normal subjects (0.22+/-0.09 mu mo l/mmol creatinine, n=11). It was also noted that urinary deoxyadenosine was below the detection limits in the ADA-deficient bone marrow transplant pat ient, but it was detected in the heterozygote (3.7 mu mol/mmol creatinine). Furthermore, it was also demonstrated that a fructose infusion increased t he urinary concentration of adenosine from 0.21+/-0.03 to 2.66+/-1.21 mu mo l/mmol creatinine in five normal subjects. (C) 1998 Elsevier Science BN. Al l rights reserved.