The enzymatic synthesis of isotopically labelled penicillin Ns with isopenicillin N synthase

Citation
Je. Baldwin et al., The enzymatic synthesis of isotopically labelled penicillin Ns with isopenicillin N synthase, J LABEL C R, 41(12), 1998, pp. 1145-1163
Citations number
19
Categorie Soggetti
Chemistry & Analysis","Inorganic & Nuclear Chemistry
Journal title
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
ISSN journal
03624803 → ACNP
Volume
41
Issue
12
Year of publication
1998
Pages
1145 - 1163
Database
ISI
SICI code
0362-4803(199812)41:12<1145:TESOIL>2.0.ZU;2-S
Abstract
The preparation of isotopically labelled penicilllin Ns using a chemico-enz ymatic approach is described. This route involves the chemical synthesis of variously labelled (D) under bar,(L) under bar,(D) under bar,-aminoadipoyl -cysteinyl-valine tripeptides via well established facile protocols and con cludes with the conversion of these tripeptides directly into penicillin Ns by the action of recombinant isopenicillin N synthase. Milligram quantitie s of isotopically labelled penicillin Ns, which would otherwise represent v ery challenging and expensive synthetic targets, are readily accessible fro m this route.