Reactivity of native conglutinin in bovine serum with rabbit antibody against recombinant bovine conglutinin with deletion of the N-terminal and collagen-like regions

The reactivity of native bovine conglutinin (Kg) with antibody against reco mbinant Kg (rKg), with deletion of the N-terminal and collagen-like regions of the native Kg molecule, was studied by sandwich enzyme-linked immunosor bent assay. With anti-recombinant Kg antibody as the coating antibody, rKg reacted with biotinylated homologous anti-rKg and heterologous anti-Kg anti bodies as probing antibodies, while native Kg did not. With anti-native Kg antibody as coating antibody, native Kg reacted with biotinylated homologou s antibody as probing antibody, while recombinant Kg reacted weakly with bo th biotinylated homologous and heterologous antibodies. Consequently the N- terminal and collagen-like regions of native Kg molecule are essential to e xpress the complete immunogenicity and/or antigenicity of the native Kg mol ecule.