P. Seifert et al., DISTRIBUTION PATTERN OF NERVOUS-TISSUE AND PEPTIDERGIC NERVE-FIBERS IN ACCESSORY LACRIMAL GLANDS, Current eye research, 16(4), 1997, pp. 298-302
Purpose. Demonstration of the distribution pattern of nervous tissue a
nd the occurrence of neuropept human accessory lacrimal glands by mean
s of immunohistochemical methods. Methods. Paraffin sections of tissue
samples were incubated with antisera against protein gene product (PG
P), S-100 protein (S-100), calcitonin gene-related peptide (CGRP) and
substance P (SP). The immunoreactions were visualized using both speci
es-specific secondary antibodies and a streptavidin-biotin-peroxidase
complex (ABC method). Results. PGP- and S-100-immunoreactive nerve fib
ers were distributed in the entire intertubular stroma. In addition, m
yoepithelial cells, a few clusters of tubulus cells, groups of wanderi
ng cells, and scattered cells of the excretory duct system were S-100-
immunoreactive. CGRP- and SP-immunoreactivity was found in a few nerve
fibers in the intertubular glandular stroma with an association to se
cretory tubules, blood vessels, intralobular ducts and excretory duct.
Conclusions. These observations are in agreement with findings in the
main lacrimal gland.