Mitochondrial oxygen consumption, lipid peroxidation and antioxidant enzyme systems in skeletal muscle of senile dystrophic mice

To evaluate age-dependent abnormalities in mitochondrial redox metabolism s pecifically in dystrophic skeletal muscle, oxygen consumption, thiobarbitur ic acid reactivity (TBARS), free-radical scavengers and oxidative marker en zymes were measured in the skeletal muscle from adult and senile control (C 57BL/10) and dystrophic (mdx/+) mice. Mitochondrial oxygen consumption in s tate 3 was significantly lowered with age in the senile dystrophic (-52%) a nd less markedly in the senile control (-30%) skeletal muscle. Compared wit h adult muscle, mitochondrial concentration of TBARS and cellular concentra tion of lipofuscin were significantly increased in senile control and dystr ophic muscle. Enzymatic activity of glutathione peroxidase (GSH-Px) and con centration of alpha-tocopherol were significantly increased in the senile c ontrol (GSH-Px 43+/-5.7 vs 53+/-8.7 U/g protein, alpha-tocopherol 0.19+/-0. 09 vs 0.29+/-0.14 mu mol/g total lipids), but significantly decreased in th e senile dystrophic (GSH-Px 80+/-8.0 vs 53+/-12 U/g protein, alpha-tocopher ol 0.45+/-0.13 vs 0.19+/-0.03 mu mol/g total lipids) muscle. Selenium conte nt was significantly decreased only in senile dystrophic muscle (1.37+/-0.4 2 vs 0.78+/-0.21 nmol/g wet muscle). In conclusion, the enzymatic adaptatio n to reactive oxygen species was limited in the dystrophic skeletal muscle, suggesting a higher need for antioxidants, especially alpha-tocopherol.