PHILADELPHIA-CHROMOSOME IN CHRONIC MYELOGENOUS LEUKEMIA - CONFIRMATION OF CYTOGENETIC DIAGNOSIS IN PH POSITIVE AND NEGATIVE CASES BY FLUORESCENCE IN-SITU HYBRIDIZATION

In this study, patients with Philadelphia (Ph) negative and positive c hronic myelogenous leukemia (CML) were analyzed by unicolor- and dual color- (DC-) fluorescence in situ hybridization (FISH) using abl and b cr cosmid probes. Unicolor- and DC-FlSH analysis revealed a BCR-ABL fu sion in a Ph-negative patient. DC-FISH to interphase nuclei revealed t he BCR-ABL fusion, even though no metaphases were available for metaph ase-FISH and conventional cytogenetic analysis. In the remaining cases , the findings of FISH were in agreement with the cytogenetic results. (C) Elsevier Science inc., 1997.