DETECTION OF TRISOMY-8 USING CONVENTIONAL CYTOGENETIC TECHNIQUES AND INTERPHASE FISH ANALYSIS IN 34 MYELOID DISORDERS - A COMPARATIVE-STUDY

Trisomy for chromosome 8 is one of the most common chromosome abnormal ities detected in myeloid disorders. However, and despite its great in cidence, its biologic significance remains poorly understood. Recently , some reports have shown the existence of masked trisomy 8 in myeloid disorders. To try to detect occult trisomy 8, we performed a study in which 34 myeloid disorders with apparent normal karyotypes using conv entional cytogenetic techniques were analyzed using fluorescence in si tu hybridization (FISH) analysis. Masked trisomy 8 was not detected in any of the 34 cases studied. This suggests that trisomy 8 may be a ra re event in myeloid disorders in which an optimal conventional cytogen etic analysis has been performed. The acquisition of trisomy 8 either in early neoplastic stages in some cases or in late neoplastic stages in others and the absence of masked trisomy 8 suggest that this chromo some alteration may not be essential to the development of myeloid dis orders. (C) Elsevier Science Inc., 1997.