EFFECT OF CONDITIONED MEDIA, NUTRITIVE ELEMENTS, AND MITOTIC SYNCHRONIZATION ON THE ACCURACY OF THE CYTOGENETIC ANALYSIS IN ACUTE NONLYMPHOCYTIC LEUKEMIA PATIENTS PRESENTING WITH INV(16) T(16-16) OR T(15-17)/

Citation
C. Castagne et al., EFFECT OF CONDITIONED MEDIA, NUTRITIVE ELEMENTS, AND MITOTIC SYNCHRONIZATION ON THE ACCURACY OF THE CYTOGENETIC ANALYSIS IN ACUTE NONLYMPHOCYTIC LEUKEMIA PATIENTS PRESENTING WITH INV(16) T(16-16) OR T(15-17)/, Cancer genetics and cytogenetics, 94(2), 1997, pp. 106-112
Citations number
33
Categorie Soggetti
Oncology,"Genetics & Heredity
ISSN journal
01654608
Volume
94
Issue
2
Year of publication
1997
Pages
106 - 112
Database
ISI
SICI code
0165-4608(1997)94:2<106:EOCMNE>2.0.ZU;2-L
Abstract
To improve the yield of the cytogenetic analysis in patients with acut e nonlymphocytic leukemia (ANLL), six culture conditions for bone marr ow or peripheral blood cells were tested in parallel. Two conditioned media (CM), phytohemagglutinin leukocyte PHA-LCM and 5637 CM, nutritiv e elements (NE), and methotrexate (MTX) cell synchronization were inve stigated in 14 patients presenting with either inv(16)/t(16;16) (group 1, n = 9 patients) or t(15;17) (group 2, n = 5). The criteria used to identify the most favorable culture conditions were the mitotic index (MI), the morphological index (MorI), and the percentage of abnormal metaphases. In the presence of PHA-LCM and 5637 CM, the MI were signif icantly increased in group 2, whereas in the MTX conditions, MI remain ed very low in both groups. The values of the MorI did not reveal any significant changes in chromosome resolution between the conditions in either group. The addition of NE did not have a positive effect in qu antity or quality of metaphases. Because of the variability of the res ponse of leukemic cells to different stimulations in vitro, several cu lture conditions in parallel are required to ensure a satisfactory yie ld of the chromosome analysis in ANLL. (C) Elsevier Science Inc., 1997 .