EFFECT OF CONDITIONED MEDIA, NUTRITIVE ELEMENTS, AND MITOTIC SYNCHRONIZATION ON THE ACCURACY OF THE CYTOGENETIC ANALYSIS IN ACUTE NONLYMPHOCYTIC LEUKEMIA PATIENTS PRESENTING WITH INV(16) T(16-16) OR T(15-17)/
C. Castagne et al., EFFECT OF CONDITIONED MEDIA, NUTRITIVE ELEMENTS, AND MITOTIC SYNCHRONIZATION ON THE ACCURACY OF THE CYTOGENETIC ANALYSIS IN ACUTE NONLYMPHOCYTIC LEUKEMIA PATIENTS PRESENTING WITH INV(16) T(16-16) OR T(15-17)/, Cancer genetics and cytogenetics, 94(2), 1997, pp. 106-112
To improve the yield of the cytogenetic analysis in patients with acut
e nonlymphocytic leukemia (ANLL), six culture conditions for bone marr
ow or peripheral blood cells were tested in parallel. Two conditioned
media (CM), phytohemagglutinin leukocyte PHA-LCM and 5637 CM, nutritiv
e elements (NE), and methotrexate (MTX) cell synchronization were inve
stigated in 14 patients presenting with either inv(16)/t(16;16) (group
1, n = 9 patients) or t(15;17) (group 2, n = 5). The criteria used to
identify the most favorable culture conditions were the mitotic index
(MI), the morphological index (MorI), and the percentage of abnormal
metaphases. In the presence of PHA-LCM and 5637 CM, the MI were signif
icantly increased in group 2, whereas in the MTX conditions, MI remain
ed very low in both groups. The values of the MorI did not reveal any
significant changes in chromosome resolution between the conditions in
either group. The addition of NE did not have a positive effect in qu
antity or quality of metaphases. Because of the variability of the res
ponse of leukemic cells to different stimulations in vitro, several cu
lture conditions in parallel are required to ensure a satisfactory yie
ld of the chromosome analysis in ANLL. (C) Elsevier Science Inc., 1997
.