Homology modeling of an RNP domain from a human RNA-binding protein: Homology-constrained energy optimization provides a criterion for distinguishingpotential sequence alignments

Citation
Pv. Sahasrabudhe et al., Homology modeling of an RNP domain from a human RNA-binding protein: Homology-constrained energy optimization provides a criterion for distinguishingpotential sequence alignments, PROTEINS, 33(4), 1998, pp. 558-566
Citations number
36
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PROTEINS-STRUCTURE FUNCTION AND GENETICS
ISSN journal
08873585 → ACNP
Volume
33
Issue
4
Year of publication
1998
Pages
558 - 566
Database
ISI
SICI code
0887-3585(199812)33:4<558:HMOARD>2.0.ZU;2-O
Abstract
We have recently described an automated approach for homology modeling usin g restrained molecular dynamics and simulated annealing procedures (Li et a l, Protein Sci,, 6:956-970,1997), We have employed this approach for constr ucting a homology model of the putative RNA-binding domain of the human RNA -binding protein with multiple splice sites (REP-MS), The regions of REP-MS which are homologous to the template protein snRNP U1A were constrained by "homology distance constraints," while the conformation of the non-homolog ous regions were defined only by a potential. energy function. A full energ y function without explicit solvent was employed to ensure that the calcula ted structures have good conformational energies and are physically reasona ble. The effects of misalignment of the unknown and the template sequences were also explored in order to determine the feasibility of this homology m odeling method for distinguishing possible sequence alignments based on con siderations of the resulting conformational energies of modeled structures. Differences in the alignments of the unknown and the template sequences re sult in significant differences in the conformational energies of the calcu lated homology models, These results suggest that conformational energies a nd residual constraint violations in these homology-constrained simulated a nnealing calculations can be used as criteria to distinguish between correc t and incorrect sequence alignments and chain folds. (C) 1998 Wiley-Liss, I nc.