Coat protein-mediated resistance to pea enation mosaic virus in transgenicPisum sativum L.

Authors
Chowrira, GM Cavileer, TD Gupta, SK Lurquin, PF Berger, PH
Citation
Gm. Chowrira et al., Coat protein-mediated resistance to pea enation mosaic virus in transgenicPisum sativum L., TRANSGEN RE, 7(4), 1998, pp. 265-271
Citations number
19
Categorie Soggetti
Molecular Biology & Genetics
Journal title
TRANSGENIC RESEARCH
ISSN journal
09628819 → ACNP
Volume
7
Issue
4
Year of publication
1998
Pages
265 - 271
Database
ISI
SICI code
0962-8819(199807)7:4<265:CPRTPE>2.0.ZU;2-R
Abstract
Pea (Pisum sativum L.) plants were transformed in planta by injection/elect roporation of axillary meristems with a chimeric pea enation mosaic virus ( PEMV) coat protein gene contruct. R-1 progenies of these plants were shown to harbor the transgene by polymerase chain reaction (PCR) and genomic Sout hern analysis, while transgene expression was demonstrated by western blot analysis. Transgenic R-2, R-3 and R-4 plants displayed delayed or transient PEMV multiplication and attenuated symptoms as compated to control inocula ted individuals.