Pea (Pisum sativum L.) plants were transformed in planta by injection/elect
roporation of axillary meristems with a chimeric pea enation mosaic virus (
PEMV) coat protein gene contruct. R-1 progenies of these plants were shown
to harbor the transgene by polymerase chain reaction (PCR) and genomic Sout
hern analysis, while transgene expression was demonstrated by western blot
analysis. Transgenic R-2, R-3 and R-4 plants displayed delayed or transient
PEMV multiplication and attenuated symptoms as compated to control inocula
ted individuals.