Anti-endomysium antibodies detection in the celiac disease screening: Indirect immunofluorescence pattern using umbilical cord sections as substrate

Celiac disease screening test with anti-endomysial antibodies detection is limited by the use of endangered species tissue (monkey distal oesophagus) as well as by the high costs of commercial kits. Due to such limitations ne w tests are developed to avoid the use of monkey tissue: an IFA test utilis ing umbilical cord sections (a discarded tissue) and an ELISA test detectin g anti-transglutaminase antibodies. In most cases umbilical cord anti-endomysium test, is not routinely used du e to the different pattern of EmA IFA test with monkey oesophagus: in the p resent article we show some pattern obtained with this new method in order to clarify possible reading misinterpretation.