Multiple sample PCR amplification and electrophoretic analysis on a microchip

Citation
Lc. Waters et al., Multiple sample PCR amplification and electrophoretic analysis on a microchip, ANALYT CHEM, 70(24), 1998, pp. 5172-5176
Citations number
18
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL CHEMISTRY
ISSN journal
00032700 → ACNP
Volume
70
Issue
24
Year of publication
1998
Pages
5172 - 5176
Database
ISI
SICI code
0003-2700(199812)70:24<5172:MSPAAE>2.0.ZU;2-J
Abstract
Polymerase chain reactions (PCRs) were carried out on as many as four DNA s amples at a time on a microchip device. The PCR products were then analyzed , either individually or together on the same device, by microchip gel elec trophoresis. A standard PCR protocol was used to amplify 199- and 500-base pair (bp) regions of bacteriophage lambda DNA and 346- and 410-bp regions o f E. coli genomic and plasmid DNAs, respectively. Thermal lysis of the bact eria was integrated into the PCR cycle. A product sizing medium, poly(dimet hylacrylamide), and an intercalating dye for fluorescence detection were us ed in the electrophoretic analysis of the products. PCR product sizes were determined by coelectrophoresis with marker DNA.