Does AK-2123 (Senazole) have sensitizing effects on radiation, cisplatin and hyperthermia under aerobic conditions in vitro?

We investigated the sensitizing effects of AK-2123 (Senazole) on the intera ction of radiation, cisplatin and hyperthermia under aerobic conditions in the rat yolk sac cell line NMT-1R in vitro. The effects were assessed by cl onogenic assay. A cytotoxic effect of AK-2123 after 24 hours exposure was o bserved as a function of the dose. I;or NMT-1R cells, the ID70 of AK-2123 w as 400 mu g/ml for 24 hours exposure, which was employed for subsequent com bined treatments. Although a statistically significant increase in the G1 c ell fraction was observed after AK-2123 treatment with a dose of ID70 (p=0. 02) no enhancing effect of AK-2123 on radiation, cisplatin or heat response curves was detected under aerobic conditions in vitro.