Construction of a bioluminescent reporter strain to detect polychlorinatedbiphenyls

Authors
Layton, AC Muccini, M Ghosh, MM Sayler, GS
Citation
Ac. Layton et al., Construction of a bioluminescent reporter strain to detect polychlorinatedbiphenyls, APPL ENVIR, 64(12), 1998, pp. 5023-5026
Citations number
36
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
64
Issue
12
Year of publication
1998
Pages
5023 - 5026
Database
ISI
SICI code
0099-2240(199812)64:12<5023:COABRS>2.0.ZU;2-I
Abstract
A bioluminescent reporter strain, Ralstonia eutropha ENV307(pUTK60), was co nstructed for the detection of polychlorinated biphenyls by inserting the b iphenyl promoter upstream of the bioluminescence genes. In the presence of a nonionic surfactant, which enhances the solubility of chlorinated bipheny ls, bioluminescence was induced three- to fourfold over background by biphe nyl, monochlorinated biphenyls, and Aroclor 1242. The minimum detection lim its for these compounds ranged from 0.15 mg/liter for l-chlorobiphenyl to 1 .5 mg/liter for Aroclor 1242.