A simple and reliable method for hybridization of homothallic wine strainsof Saccharomyces cerevisiae

A procedure was developed for the hybridization and improvement of homothal lic industrial wine yeasts. Killer cycloheximide-sensitive strains were cro ssed with killer-sensitive cycloheximide-resistant strains to get killer cy cloheximide-resistant hybrids, thereby enabling hybrid selection and identi fication. This procedure also allows backcrossing of spore colonies from th e hybrids with parental strains.