Ch. Kuhner et al., SPOROMUSA SILVACETICA SP-NOV, AN ACETOGENIC BACTERIUM ISOLATED FROM AGGREGATED FOREST SOIL, International journal of systematic bacteriology, 47(2), 1997, pp. 352-358
Sporomusa silvacetica sp. nov. DG-1(T) (= DSMZ 10669(T)) (T = type str
ain) was isolated from well-drained, aggregated forest soil (pH 6.0) i
n east-central Germany. The cells were obligately anaerobic, slightly
curved rods and were motile by means of laterally inserted flagella on
the concave side of each cell. Typical cells were approximately 3.5 b
y 0.7 mu m. Cells stained weakly gram positive, but thin sections reve
aled a complex multilayer cell wall. Spores were spherical and distend
ed the sporangia. Growth and substrate utilization occurred with ferul
ate, vanillate, fructose, betaine, fumarate, 2,3-butanediol, pyruvate,
lactate, glycerol, ethanol, methanol, formate, and H-2-CO2. With most
substrates, acetate was the primary reduced end product and was produ
ced in stoichiometries indicative of an acetyl-coenzyme a pathway-depe
ndent metabolism. Fumarate was dismutated to succinate and acetate. Me
thoxyl and acrylate groups of various aromatic compounds were O-demeth
ylated and reduced, respectively. Yeast extract was not required for g
rowth. Cells grew optimally at approximately 30 degrees C and pH 6.8;
under these conditions and with fructose as the substrate the doubling
time was approximately 14 h. The lowest temperature that supported gr
owth was between 5 and 10 degrees C. The carbon monoxide dehydrogenase
and hydrogenase activities were approximately 9 and 102 mu mol min(-1
) mg of protein(-1), respectively. A type b cytochrome was detected in
the membrane. The G+C content was approximately 43 mol%. Phylogenetic
analysis of the 16S ribosomal DNA indicated that DG-1(T) was most clo
sely related to members of the genus Sporomusa in the clostridium subp
hylum of the gram-positive bacteria.