Determination of aloesin in plasma by high-performance liquid chromatography as fluorescent 9-anthroyl derivative

A sensitive high-performance liquid chromatographic (HPLC) method for the d etermination of aloesin in plasma was developed. After solid-phase extracti on from plasma and derivatization of aloesin and compound AD-I, which was p repared from aloesin asa internal standard, with 9-anthroylnitrile in the p resence of quinuclidine, the derivatives were separated on a Inertsil ODS-3 column using acetonitrile/methanol/water (3:1:6) as a mobile phase, and de tected fluorimetrically at 460 nm with excitation at 360 nm. The detection limit of aloesin was 3.2 ng/ml in plasma (S/N=3).