Adsorption of fibrinogen to droplets of liquid hydrophobic phases - Functionality of the bound protein and biological implications

Fibrinogen adsorbs spontaneously from aqueous media containing that protein to droplets of liquid hydrophobic phases dispersed in those same media. Ex amples of such phases include mineral oils, straight-chain hydrocarbons, an d various plant- and animal-derived oils. Lecithin preexisting on the surfa ce of oil droplets reduces significantly the amount of fibrinogen that can otherwise bind to them. When bound, fibrinogen remains active in the classi c sense of fibrin gelation. As a consequence, oil droplets coated with fibr inogen can participate in a host of biologically important adhesive process es in which the protein would be expected to participate. Certain polyanion s, eg, heparin, pentosan polysulfate, dextran sulfate, and suramin, bind to adsorbed fibrin(ogen) and prevent thrombin-dependent adhesion of fibrinoge n-coated surfaces. Thus, these polyanions can be used to prevent adhesion b etween fibrin(ogen)-coated oil droplets and other fibrin(ogen)-coated surfa ces. Potential practical applications and biological implications of these phenomena are presented and discussed.