JANIBACTER LIMOSUS GEN-NOV, SP-NOV, A NEW ACTINOMYCETE WITH MESO-DIAMINOPIMELIC ACID IN THE CELL-WALL

New gram-positive bacteria were isolated from 1-year-old sludge from a wastewater treatment plant, The isolates are coccoid to rod-shaped, n onmotile aerobes that form neither spores nor mycelia. They are charac terized by a peptidoglycan with directly cross-linked meso-diaminopime lic acid (type A1 gamma), by the presence of menaquinone MK-8(H-4), an d by the lack of mycolic acids, The strains have complex fatty acid pa tterns with i-C-16:0 and straight-chain saturated and unsaturated fatt y acids as major components, The G+C content of the DNA is 70 mol%. Th e results of chemotaxonomic studies and a 16S ribosomal DNA sequence c omparison support our proposal to assign these bacteria to a new genus , the genus Janibacter gen, nov.; the type species is Janibacter limos us sp. nov., and the type strain of J. limosus is strain HKI 83 (= DSM 11140).