Spectrophotometric quantitative analysis of the main hemoglobin derivatives

A spectrophotometric multiwavelength method for the simultaneous determinat ion of the main hemoglobin derivatives including oxyhemoglobin, deoxyhemogl obin, carboxyhemoglobin, and methemoglobin has been developed. The choice o f analytical wavelengths was made by the methods of information factors and linear programming. The software for the quantitative analysis was develop ed considering the least square method, linear programming, algebraic backg round correction; and combined methods including linear programming and alg ebraic background correction.