R. Abonour et al., Autologous transplantation of mobilized peripheral blood CD34(+) cells selected by immunomagnetic procedures in patients with multiple myeloma, BONE MAR TR, 22(10), 1998, pp. 957-963
Citations number
33
Categorie Soggetti
Hematology,"Medical Research Diagnosis & Treatment
In the use of autologous PBPC transplantation in patients with multiple mye
loma, contamination of PBPC,vith myeloma cells is commonly observed. Enrich
ment for CD34(+) cells has been employed as a method of reducing this conta
mination, In this study the reduction of myeloma cells in PBPC was accompli
shed by the positive selection of CD34(+) cells using immunomagnetic bead s
eparation (Isolex 300 system). PBPC were mobilized from 18 patients using c
yclophosphamide (4.5 g/m(2)) and G-CSF (10 mu g/kg/day), A median of two le
ukaphereses and one selection was performed per patient. The median number
of mononuclear cells processed was 3.50 x 10(10) with a recovery of 1.11 x
10(8) cells after selection, The median recovery of CD34(+) cells was 48% (
range 17-78) and purity was 90% (29-99), The median log depletion of CD19() cells was 3.0, IgH rearrangement, assessed by PCR, was undetectable in 13
of 24 evaluable CD34(+) enriched products. Patients received 200 mg/m(2) o
f melphalan followed by the infusion of a median of 2.91 x 10(6)/kg CD34(+)
cells (1.00-16.30), The median time to absolute neutrophil count >0.5 x 10
(9)/1 was 11 days, and sustained platelet recovery of >20 x 10(9)/1 was 14
days, We conclude that immunomagnetic-based enrichment of CD34(+) cells res
ults in a marked reduction in myeloma cells without affecting engraftment k
inetics.