Time course of superoxide generation in reperfusion after arterial ischaemia and venous congestion

Citation
K. Nakamura et al., Time course of superoxide generation in reperfusion after arterial ischaemia and venous congestion, BR J PL SUR, 51(8), 1998, pp. 633-636
Citations number
20
Categorie Soggetti
Surgery
Journal title
BRITISH JOURNAL OF PLASTIC SURGERY
ISSN journal
00071226 → ACNP
Volume
51
Issue
8
Year of publication
1998
Pages
633 - 636
Database
ISI
SICI code
0007-1226(199812)51:8<633:TCOSGI>2.0.ZU;2-7
Abstract
In order to identify differences in superoxide (O-2(-)) production after re perfusion following arterial ischaemia and venous congestion, we directly q uantified O-2(-) in the venous effluent from reperfused hindlimbs in the ra bbit. Using a total of 20 rabbit hindlimbs, we made hindlimb-reperfusion mo dels by section of both soft tissue and bony structures exclusive of femora l vessels around the thighs and clamping of the artery alone (group A: n = 10) or vein alone (group V: n = 10) by microvascular clamp. A pilot study r evealed that the critical time for venous congestion was 2 h. Accordingly, clamping was performed at 25 degrees C (normothermia) for 2 h. Sham operati ons were performed in the control (n = 7). Venous effluent blood samples fr om the femoral vein proximal to the clamping point were collected before re perfusion, and from 5 to 60 min after reperfusion at 5 min intervals. Efflu ent blood samples were used to quantify O-2(-) using a chemiluminescence me thod using a derivative of luciferin. The time course of O-2(-) production after reperfusion in group A and group V showed significant differences (P < 0.05), compared with the controls. There was no significant difference in the time course of O-2(-) production in group A and that in group V. In th e early reperfusion phase, no difference in O-2(-) production was observed after arterial ischaemia or venous congestion. In future studies, we will t est the contributions of free radicals by setting longer ischaemic times, l onger reperfusion times, and studying secondary ischaemia.