Quantitative reverse transcription PCR assay of the RNA component of humantelomerase using the TaqMan fluorogenic detection system

Authors
Yajima, T Yagihashi, A Kameshima, H Kobayashi, D Furuya, D Hirata, K Watanabe, N
Citation
T. Yajima et al., Quantitative reverse transcription PCR assay of the RNA component of humantelomerase using the TaqMan fluorogenic detection system, CLIN CHEM, 44(12), 1998, pp. 2441-2445
Citations number
20
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
CLINICAL CHEMISTRY
ISSN journal
00099147 → ACNP
Volume
44
Issue
12
Year of publication
1998
Pages
2441 - 2445
Database
ISI
SICI code
0009-9147(199812)44:12<2441:QRTPAO>2.0.ZU;2-Q
Abstract
We established the validity of a quantitative reverse transcription (RT)-PC R assay for the RNA component of human telomerase (hTR), using the TaqMan f luorogenic detection system. Using this assay, we quantified hTR expression in two human pancreatic cancer cell lines, ASPC-1 and MIAPaCa-2. Our resul ts indicated that hTR expression in MIAPaCa-2 was 1.99-fold higher than tha t in ASPC-1 cells. This TaqMan RT-PCR assay appears to be useful in determi ning the amount of hTR in clinical specimens.