EPSTEIN-BARR VIRUS-SPECIFIC CYTOTOXIC T-CELL RESPONSES IN HIV-1 INFECTION - DIFFERENT KINETICS IN PATIENTS PROGRESSING TO OPPORTUNISTIC INFECTION OR NON-HODGKINS-LYMPHOMA

Although the high incidence of EBV-associated diffuse large cell lymph omas (DLCL) in HIV-1 infection is believed to be related to loss of im mune control due to HIV-induced immune deficiency, it has been claimed that cytotoxic T lymphocyte (CTL) responses to EBV are longer lasting in HIV-l-infected persons than CTL directed against HIV-1 itself, We approached this apparent paradox by performing the first longitudinal study into the kinetics of EBV and HIV-specific CTL responses in HIN-i nfected patients progressing either to AIDS with non-Hodgkin's lymphom a (NHL) or AIDS with opportunistic infection (OI), Multiple samples we re tested from HIV-1 seroconversion to AIDS-diagnosis. Four out of six patients that were either long-term asymptomatic or progressing to OI showed declining HIV-1 CTL precursor (CTLp) frequencies whereas EBV-C TLp remained stable, suggestive for HIV-l-specific immune exhaustion, In two patients rapidly progressing to AIDS-OI, a parallel decline of HIV-1- and EBV-CTL responses was seen, indicative for total collapse o f cellular immunity, In all these six patients EBV-load remained low, However, in four out of five patients that progressed to DLCL, EBV-loa d was high and increasing several months preceding the NHL. In all fiv e patients, EBV-CTLp decreased before the emergence of the NHL, Thus, our data show that in HIV-1 infection loss of HIV-1-specific T cell im munity is not necessarily paralleled by loss of EBV-specific T cell re sponses, The occurrence of AIDS-related DLCL is preceded by decreasing EBV-CTLp and increasing EBV load, Failing EBV-control might therefore be an important step in the pathogenesis of AIDS-related DLCL.