Resistance to alfalfa mosaic virus in transgenic burley tobaccos expressing the AMV coat protein gene

There are no known genetic sources of resistance to alfalfa mosaic virus (A MV) in the genus Nicotiana. In this communication, we describe holy we gene tically engineered an AMV (strain 425) coat protein (CP) gene into three co mmercial burley tobacco (N. tabacum L.) genotypes, BY 21, TN 86, and KY 895 9, and the evaluation of the transgenic lines in greenhouse and field exper iments. In a replicated greenhouse trial, 11 AMV-CP transgenic lines in a B Y 21 backgound were resistant to two AMV isolates, AMV-425 and AMV-KY, but the lines were less resistant to another isolate, AMV-NC. In a 1994 field e xperiment, we found that nine BY 21 AMV-CP transgenic lines were resistant to AMV-KY when inoculated both before and after transplanting. The level of resistance varied among the nine lines. In 1995, 10 randomly selected AMV- CP lines in each of the three genetic backgrounds were evaluated in a repli cated field trial. Mean infection scores of each set of lines were signific antly less than those of their non-transgenic counterparts, but there were no significant differences among these three mean infection scores. However , there were significant differences among Lines within a genetic backgroun d for their response to AMV infection. High sensitivity to AMV infection, f ound in genotypes TN 86 and KY 8959 that possess an endogenous gene for res istance to potyviruses, was not evident in their AMV-CP transgenic lines. T he CP mediated resistance to AMV was effective in reducing viral symptoms i n the field and it may provide a valuable source far AMV resistance in toba cco.