DNA-based markers for a recessive gene conferring anthracnose resistance in sorghum

Resistance to anthracnose in sorghum [Sorghum bicolor (L.) Moench] accessio n SC326-6 was found to segregate as a simple recessive trait in a cross to the susceptible cultivar BTx623, F3 progeny tests following self pollinatio n of 115 F-2 individuals identified homozygous resistant and susceptible F- 2 plants for use in bulked-segregant analysis. DNA from the parental cultiv ars and the bulks was screened by PCR amplification with 300 RAPD (random a mplified polymorphic DNA) primers. Two RAPD primers amplified a sequence th at co-segregated with the recessive resistance allele, while another amplif ied a band linked to the susceptible allele.