The effect of dimethylsulfoxide on the water transport response of rat liver tissue during freezing

Citation
Dj. Smith et al., The effect of dimethylsulfoxide on the water transport response of rat liver tissue during freezing, CRYO-LETT, 19(6), 1998, pp. 343-354
Citations number
31
Categorie Soggetti
Experimental Biology
Journal title
CRYO-LETTERS
ISSN journal
01432044 → ACNP
Volume
19
Issue
6
Year of publication
1998
Pages
343 - 354
Database
ISI
SICI code
0143-2044(199811/12)19:6<343:TEODOT>2.0.ZU;2-S
Abstract
This work investigates the biophysical response of water transport in a cli nically important tissue type-liver-to freezing in the presence of dimethyl sulfoxide (DMSO). Sprague-Dawley rat liver tissue samples were frozen in RP MI media supplemented with 1 and 2 M DMSO at a cooling rate of 5 degrees C/ min. The water transport was measured by cell volumetric changes as assesse d by low temperature freeze substitution microscopy and image analysis. The se data were compared to a previous study where liver in the absence of DMS O was cooled at the same rate and shown to be completely dehydrated at -20 degrees C [27]. Curve-fitting a water transport model to the experimental d ata showed that L-pg and E-Lp drop from 1.9 mu m/min-atm and 69 kcal/mole i n the absence of DMSO [27] to 0.033 mu m/min-atm and 8.1 kcal/mol in 2 M DM SO. The available data were unable to support a statistically significant d ifference between the 1 M and 2 M DMSO parameters.