Electrooxidation of aliphatic amines and their amperometric detection in flow injection and liquid chromatography at a nickel-based glassy carbon electrode

The electrocatalytic oxidation of mono- and poly-amines was investigated in alkaline solutions at a nickel-based chemically modified glassy carbon ele ctrode. beta-NiOOH exhibited electrocatalytic activity towards amines oxida tion. FIA experiments were performed to test the electrode as an amperometr ic sensor. Under constant-potential amperometric mode (at 0.55 V vs. 4,0 M Ag/AgCl/Cl-), detection limits of all investigated analytes ranged within 1 .2-3.8 pmol (S/N=3), and the linear dynamic range extended over three order s oi magnitude above the detection limits. Chromatographic separations, per formed by an ion moderated partition column in alkaline solutions equipped with the Ni-CME detector, allowed very simple quantitation of aliphatic ami nes, avoiding tedious and time-consuming derivatization processes or postco lumn addition procedures. The Ni-CME showed good temporal stability and app reciable sensitivity.