H. Watzig et al., Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications, ELECTROPHOR, 19(16-17), 1998, pp. 2695-2752
This review is in support of the development of selective, reproducible and
validated capillary electrophoretis (CE) methods. Focusing on pharmaceutic
al and biological applications, the successful use of CE is demonstrated by
more than 800 references, mainly from 1994 until 1998. Approximately 80 re
cent reviews have been catalogued. These articles sum up the existing strat
egies for method development in CE, especially in the search for generally
accepted concepts, but also looking for new, promising reagents and ideas.
General strategies for method development were derived not only with regard
to selectivity and efficiency, but also with regard to precision, short an
alysis time, limit of detection, sample pretreatment requirements and valid
ation. Standard buffer recipes, surfactants used in micellar electrokinetic
capillary chromatography (MEKC), chiral selectors, useful buffer additives
, polymeric separation media, electroosmotic flow (EOF) modifiers, dynamic
and permanent coatings, actions to deal with complex matrices and aspects o
f validation are collected in 20 tables. Detailed schemes for the developme
nt of MEKC methods and chiral separations, for optimizing separation effici
ency, means of troubleshooting, and other important information for key dec
isions during method development are given in 19 diagrams. Method developme
nt for peptide and protein separations, possibilities to influence the EOF
and how to stabilize it, as well as indirect detection are considered in sp
ecial sections.