Analyte identification in capillary electrophoretic separation techniques

Citation
Sj. Kok et al., Analyte identification in capillary electrophoretic separation techniques, ELECTROPHOR, 19(16-17), 1998, pp. 2753-2776
Citations number
313
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
01730835 → ACNP
Volume
19
Issue
16-17
Year of publication
1998
Pages
2753 - 2776
Database
ISI
SICI code
0173-0835(199811)19:16-17<2753:AIICES>2.0.ZU;2-C
Abstract
A review on applications of ion-line hyphenation in capillary electrophores is and capillary electrochromatography for the identification of migrating analytes is presented. There is an urgent need for unambiguous analyte iden tification by combining spectral information and observed migration times, because the parameters influencing the migration times and separation effic iencies in these separation techniques are not easily controlled, especiall y when real samples containing unknown interferences have to be analyzed. T he spectrometric techniques covered here are ultraviolet and visible radiat ion (UV/Vis) absorption, fluorescence including fluorescence line-narrowing spectroscopy, Raman spectroscopy, nuclear magnetic resonance and mass spec trometry. Attention is essentially confined to literature reports in which the extra information provided by the detector is really used for identific ation purposes, especially in real-life samples, while the interfacing as s uch and analyte detectabilities in standard solutions are only briefly disc ussed. This article covers an extensive fraction of the literature publishe d on this topic until the beginning of 1998.