Genomic aberrations in renal cell carcinomas detected by restriction landmark genomic scanning

In order to reveal and characterise genetic events occurring in renal tumor igenesis, samples of sporadic renal cell carcinomas (RCCs) were examined us ing restriction landmark genomic scanning (RLGS), an electrophoretic separa tion technique which detects gene amplification and deletion. We were able to find two fragments frequently amplified and 10 others commonly showing r educed signal intensity within the 16 tumour samples analysed. These altere d spots were located on chromosomes 2, 3, 9-12, 16, 17 and 18 according to chromosomal assigned RLGS;. A subset of reduced fragments appeared to be co rrelated to tumour type and were located within a new chromosomal region, s uggesting genetic specificity within the process of renal carcinogenesis. ( C) 1998 Elsevier Science Ltd. All rights reserved.