Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens

Authors
Tanaka, H Degawa, M Kawata, E Hayashi, J Shoyama, Y
Citation
H. Tanaka et al., Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens, FOREN SCI I, 97(2-3), 1998, pp. 139-153
Citations number
45
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology
Journal title
FORENSIC SCIENCE INTERNATIONAL
ISSN journal
03790738 → ACNP
Volume
97
Issue
2-3
Year of publication
1998
Pages
139 - 153
Database
ISI
SICI code
0379-0738(19981109)97:2-3<139:IOCPUA>2.0.ZU;2-L
Abstract
Cannabis pollen allergens were detected using the serum of an allergic pati ent. The allergens were then purified by sequential column chromatography ( including DE52 cellulose and phenyl-Sepharose CL-4B) and preparative HPLC. The molecular weight of the allergens were determined as 10 050 and 13 706 by matrix-assisted laser desorption/ionization time of flight mass spectrom etry. We utilised Western blotting and development of an enzyme-linked immu nosorbent assay for the detection of Cannabis pollen allergens. (C) 1998 El sevier Science Ireland Ltd All rights reserved.