Globin chain synthesis analysis by high performance liquid chromatography in the screening of thalassemia syndromes

We applied reversed phase high performance liquid chromatography for globin chain synthesis analysis in screening for beta-thalassemia. The alpha/non- alpha-globin chain synthesis ratios have been determined in alpha-, beta-, and delta beta-thalassemia carriers using the classical carboxymethyl cellu lose chromatography as the reference method. Reversed phase high performanc e liquid chromatography is fast, accurate, and reproducible, and may be a s uitable alternative for the traditional carboxymethyl cellulose chromatogra phy.