An efficient method for rooting and acclimation of micropropagated apple cultivars

To root tissue-cultured apple cultivars, shoots from proliferating cultures were first transferred to root induction medium with IBA for 1 week in the dark. Shoots were later transferred to the same medium without TEA and inc ubated under light for elongation of the roots. Rooted shoots were then tra nsferred to Jiffy-7s supplemented with biological plant protectant and fert ilizer, and incubated in plastic humidity trays. After 2 to 3 weeks, plants were transferred to pots and covered with plastic bags to facilitate accli mation. This technique has resulted in 70% to 100% of shoots selected in vi tro producing vigorously growing, healthy plants in the greenhouse. Chemica l name used: indolebutyric acid (IBA).