Borate complexation and mixed cyclodextrin additives for chiral separationof propranolol and its metabolites by capillary electrophoresis

A buffer system of berate with charged (carboxymethyl-beta-CD) and uncharge d (beta-CD) cyclodextrins (CDs) was employed in the chiral resolution and s eparation of propranolol and its selected major metabolites. By appropriate choice of buffer and additive conditions, chiral resolution of all of the compounds studied was achieved in a single analysis, where near baseline re solution was found for the difficult to resolve propranolol-glycol (Pr-glyc ol). This has not been observed in previous studies of propranolol and its metabolites.