The production of two Th2 cytokines, interleukin-4 and interleukin-10, is controlled independently by locus Cypr1 and by loci Cypr2 and Cypr3, respectively
M. Kosarova et al., The production of two Th2 cytokines, interleukin-4 and interleukin-10, is controlled independently by locus Cypr1 and by loci Cypr2 and Cypr3, respectively, IMMUNOGENET, 49(2), 1999, pp. 134-141
The strains BALB/cHeA (BALB/c) and STS/A (STS) differ in production of IL-4
and IL-10, two Th2 cytokines, after stimulation of spleen cells with Conca
navalin A, STS being a low and BALB/c a high producer. We analyzed the gene
tic basis of this strain difference using thp recominant congenic (RC) stra
ins of the BALB/c-c-STS/Dem (CcS/Dem) series. This series comprises 20 homo
zygous strains, Each CcS/Dem strain contains a different, random set of app
roximately 12.5% genes of the "donor" strain STS and approximately 87.5% of
the "background" strain BALB/c. We selected for further analysis the RC st
rain production intermediate between BALB/c and STS. In (CcS/20xBALB/c)F-2
hybrids we found that different loci control expression of IL-4 and IL-10.
Cyprl (cytokine production 1) on chromosome 16 near D16Mit15 controls IL-4
production, whereas the production of IL-10 is influenced by loci Cypr2 nea
r DlMit14 and DlMit227 on chromosome 1 and Cypr3 marked by D5Mit20 on chrom
osome 5. In addition, the relation;ship between the level of these two cyto
kines depends on the genotype of the F-2 hybrids at a locus coral (correlat
ion 1) on chromosome 5. This differential genetic regulation may be relevan
t for the understanding of biological effects of T-helper cells in mice of
different genotypes.