The production of two Th2 cytokines, interleukin-4 and interleukin-10, is controlled independently by locus Cypr1 and by loci Cypr2 and Cypr3, respectively

Citation
M. Kosarova et al., The production of two Th2 cytokines, interleukin-4 and interleukin-10, is controlled independently by locus Cypr1 and by loci Cypr2 and Cypr3, respectively, IMMUNOGENET, 49(2), 1999, pp. 134-141
Citations number
38
Categorie Soggetti
Immunology
Journal title
IMMUNOGENETICS
ISSN journal
00937711 → ACNP
Volume
49
Issue
2
Year of publication
1999
Pages
134 - 141
Database
ISI
SICI code
0093-7711(199902)49:2<134:TPOTTC>2.0.ZU;2-Z
Abstract
The strains BALB/cHeA (BALB/c) and STS/A (STS) differ in production of IL-4 and IL-10, two Th2 cytokines, after stimulation of spleen cells with Conca navalin A, STS being a low and BALB/c a high producer. We analyzed the gene tic basis of this strain difference using thp recominant congenic (RC) stra ins of the BALB/c-c-STS/Dem (CcS/Dem) series. This series comprises 20 homo zygous strains, Each CcS/Dem strain contains a different, random set of app roximately 12.5% genes of the "donor" strain STS and approximately 87.5% of the "background" strain BALB/c. We selected for further analysis the RC st rain production intermediate between BALB/c and STS. In (CcS/20xBALB/c)F-2 hybrids we found that different loci control expression of IL-4 and IL-10. Cyprl (cytokine production 1) on chromosome 16 near D16Mit15 controls IL-4 production, whereas the production of IL-10 is influenced by loci Cypr2 nea r DlMit14 and DlMit227 on chromosome 1 and Cypr3 marked by D5Mit20 on chrom osome 5. In addition, the relation;ship between the level of these two cyto kines depends on the genotype of the F-2 hybrids at a locus coral (correlat ion 1) on chromosome 5. This differential genetic regulation may be relevan t for the understanding of biological effects of T-helper cells in mice of different genotypes.