Simian T-lymphotropic virus type I infection among wild-caught Indonesian pig-tailed macaques (Macaca nemestrina)

Evidence for the presence of simian T-lymphotropic viruses (STLV-I) was ide ntified in Live-caught pig-tailed macaques from two locations in southern S umatra, Indonesia. Of 60 animals tested, 13.3% of the animals showed serore activity to HTLV rm enzyme-linked immunosorbent assay (ELISA) antigens. Of these, 75% showed indeterminate reactivity and 25% showed positive reactivi ty to HTLV-I/II Western blot antigens. Polymerase chain reaction (PCR) anal ysis of 6 of 8 seroreactive mon keys' peripheral blood mononuclear cell (PB MC) DNA showed production of proper size molecular weight product that hybr idized specifically to an STLV-I tax gene-specific probe. Phylogenic analys es of tau gene fragment sequences from the PCR products of two samples, 930 287 and 930306, indicated that these animals were infected with retroviruse s related to those of the Asian STLV-I clade.