The effect of the number of daily meals on milk yield and composition was s
tudied in a commercial dairy herd. One hundred and eighty Israeli Holstein
cows were divided into two groups. One group was given one meal daily and t
he second group three meals daily. Feeding frequency had no effect on DM in
take, milk yield or milk composition. Total VFA and molar proportion of ace
tate were higher before feeding than 3 h later. The decrease in molar propo
rtion of acetate after feeding was greater in the cows given one meal daily
. The propionate molar proportion was higher before feeding and lower 3 h l
ater if cows were given three meals daily. As a result, the acetate to prop
ionate ratio was lower in cows fed three meals daily before feeding and ros
e after 3 h. Ruminal ammonia-N concentrations, before and after feeding, we
re lower when cows were fed three meals daily. Plasma urea-N concentrations
were higher before feeding, as compared with 3 h later. Plasma urea-N was
higher for the one meal treatment, but significantly only 3 h after the mor
ning meal. It is suggested that increasing feeding frequency may improve di
etary nitrogen utilization and may shift metabolism towards more gluconeoge
nesis. Production responses to more frequent feeding are more likely to be
revealed in high yielding dairy cows, maintained on high concentrate diets.