Novel transitions in MHC isoforms: separate and combined effects of thyroid hormone and mechanical unloading

Single fiber (n = 3,818 fibers) electrophoretic analyses were used to delin eate the separate and combined effects of hyperthyroidism (T-3) and hindlim b suspension (HS) on the myosin heavy chain (MHC) isoform composition (1-, 2-, and 4-wk time points) of the rat soleus muscle. The key findings of thi s study are as follows. First, T-3 and HS both altered the distribution of MHC isoforms at the single-fiber level; however, the populations of fibers produced by these two interventions were clearly different from one another . Second, T-3 + HS rapidly converted the soleus into a fast muscle, produci ng large increases in the relative contents of the fast type IIx and IIb MH C isoforms which were primarily expressed in several populations of hybrid fibers (e.g., types I/IIa/IIx, I/IIx/IIb, I/IIa/lIx/IIb). Finally, T-3 + HS produced unique populations of hybrid fibers that did not adhere to the I< ->IIa<->IIx<->IIb sequential scheme of MHC plasticity. Collectively, the fi ndings of this study demonstrate that the intervention of T-3 + HS is a pow erful model for manipulating and studying MHC isoform plasticity in slow sk eletal muscle.