Centrifugal pulsing increases the recovery of viable spermatozoa

Purpose: Our purpose was to compare the number and quality of spermatozoa r ecover following the standard centrifugation method with those after pulsin g the relative centrifugal field during centrifugation. Methods: A prospectively controlled in vitro study using ejaculates from IO healthy sperm donors was undertaken. Number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5 degrees C in TEST-yolk results determ ined the efficacy of the centrifugation techniques. Results: The number of sperm and sperm motility at 0, 3, and 24 hr post-inc ubation at 5 degrees C in TEST-yolk were significantly higher for the sperm recovered following pulse centrifugation compared with those after standar d centrifugation. Conclusions: Pulsing the relative centrifugal field during centrifugation y ields a higher number of viable spermatozoa.