Tc. Marlovits et al., Soluble LDL minireceptors - Minimal structure requirements for recognitionof minor group human rhinovirus, J BIOL CHEM, 273(50), 1998, pp. 33835-33840
Soluble human low density lipoprotein minireceptors with less than seven li
gand binding repeats las are present in the native membrane receptor) were
expressed in Sf9 insect cells with a hexa-His tag fused to the C terminus.
The recombinant truncated proteins were affinity purified from the tissue c
ulture supernatants by Ni-NTA column chromatography. Minireceptors with mor
e than two repeats bound to rabbit beta very low density lipoprotein and co
uld thus be further purified by affinity chromatography. Binding and cell p
rotection assays indicated that two ligand binding repeats are sufficient f
or attachment of minor group human rhinoviruses to immobilized receptors, w
hereas at least three ligand binding repeats are required to protect HeLa c
ells against viral infection.