Reconstitution of yeast RNA polymerase I transcription in vitro from purified components - TATA binding protein is not required for basal transcription
J. Keener et al., Reconstitution of yeast RNA polymerase I transcription in vitro from purified components - TATA binding protein is not required for basal transcription, J BIOL CHEM, 273(50), 1998, pp. 33795-33802
Five purified protein components, RNA polymerase I, Rrn3p, core factor, TBP
(TATA-binding protein), and upstream activation factor, are sufficient for
high level transcription in vitro from the Saccharomyces cerevisiae rDNA p
romoter. Rrn3p and pol I form a complex in solution that is active in speci
fic initiation. Three protein components, pol I, Rrn3p, and core factor, an
d promoter sequence to -38, suffice for basal transcription. Unlike pol II
and pol III, yeast pol I basal transcription does not require TBP. Instead,
TBP, upstream activation factor, and the upstream. element of the promoter
together stimulate pol I basal transcription to a fully activated level. T
he role of TBP in pol I transcription is fundamentally different from its r
ole in pol II or pol III transcription.