Sa. Reynolds et al., Staging of middle and late embryonic development in the medicinal leech, Hirudo medicinalis, J COMP NEUR, 402(2), 1998, pp. 155-167
We present a description of the last half of embryonic development in the E
uropean medicinal leech, Hirudo medicinalis, based entirely on externally v
isible morphological features, and establish reliably observable stages dur
ing that development. Embryogenesis, from the time fertilized eggs are depo
sited in an eggcase (called a cocoon) to the emergence of juveniles from th
e cocoon, takes approximately 4 weeks at roam temperature. The stages descr
ibed in this paper extend from the completion of segmentation to the appear
ance of the final bands of pigmentation. Developmental stages are expressed
as percentages of total embryonic developmental time. This staging table w
as constructed for embryos kept at 20 degrees C. In addition, the developme
nt of animals kept at 17 degrees C or at 24 degrees C was compared with tho
se held at 20 degrees C. Development proceeds more quickly at higher temper
atures. Because development in embryos held at higher or lower temperatures
was linearly related to the stages determined for embryos held at 20 degre
es C, the rate of development at any intermediate temperature can be predic
ted from the staging table at 20 degrees C by simple multiplication. J. Com
p. Neurol. 402:155-167, 1998. (C) 1998 Wiley-Liss, Inc.