Stability and diversity of T cell receptor repertoire usage during lymphocytic choriomeningitis virus infection of mice

Numerous studies have examined T cell receptor (TCR) usage of selected viru s-specific T cell clones, yet little information is available regarding the stability and diversity of TCR repertoire usage during viral infections. H ere, we analyzed the V beta 8.1 TCR repertoire directly ex vivo by compleme ntarity-determining region 3 (CDR3) length spectratyping throughout the acu te lymphocytic choriomeningitis virus (LCMV) infection, into memory, rind u nder conditions of T cell clonal exhaustion. The V beta 8 population repres ented 30-35% of the LCMV-induced CD8(+) T cells and included T cells recogn izing several LCMV-encoded peptides, allowing for a comprehensive study of a multiclonal T cell response against a complex antigen. Genetically identi cal mice generated remarkably different T cell responses, as reflected by d ifferent spectratypes and different TCR sequences in same sized spectratype bands; however, a conserved CDR3 motif was found within some same sized ba nds. This indicated that meaningful studies on the evolution of the T cell repertoire required longitudinal studies within individual mice. Such longi tudinal studies with peripheral blood lymphocyte samples showed that (a) th e virus-induced T cell repertoire changes little during the apoptosis perio d after clearance of the viral antigens; (b) the LCMV infection dramaticall y skews the host T cell repertoire in the memory state; and (c) continuous selection of the T cell repertoire occurs under conditions of persistent in fections.