Dietary fat clearance is modulated by genetic variation in apolipoprotein A-IV gene locus

Previous studies have shown that the A-IV-347Ser polymorphism is associated with the variability in low density lipoprotein (LDL)-cholesterol response to dietary therapy, The present study was designed to evaluate the associa tion of this polymorphism with the individual variability observed in postp randial lipemic response, This polymorphism was characterized in 50 healthy male subjects homozygous for the apolipoprotein (apo)E3 allele. All subjec ts were subjected to a vitamin A-fat load test. Blood was drawn at time 0 a nd every hour over a period of 11 hours. Cholesterol and triglycerides (TG) in plasma and lipoprotein fractions of CH, TG, and retinyl palmitate (RP) were determined, Data from the postprandial lipemia revealed that subjects with the A-IV-347Ser allele (n = 14) have a lower postprandial response in total TG (P < 0.025), large triglyceride rich lipoproteins (TRL) TG (P < 0. 02), and small-TRL TG levels (P < 0.007), and a higher postprandial respons e in large-TRZ, apoA-IV (P < 0.006) and apoB-100 (P < 0.041) levels than su bjects homozygous for the A-TV-347Thr subjects (n = 36). In conclusion, the modifications observed in postprandial lipoprotein metabolism associated w ith this polymorphism within the apoA-IV gene locus may be involved in the variability in LDL-CH response observed in subjects consuming high saturate d fat diets.