A modified HPLC method for the determination of vancomycin in plasma and tissues and comparison to FPIA (TDX)

A modified high performance liquid chromatography (HPLC) method for the qua ntification of vancomycin levels in plasma and tissues is described. The me thod uses solid phase extraction (SPE) of vancomycin from the samples and r eversed phase HPLC with UV detection. The method was fully validated in ter ms of recovery, linearity, selectivity and various stability conditions. Va ncomycin was determined in plasma samples obtained from 15 patients undergo ing cardiopulmonary bypass, before and repeatedly during 12 h after drug ad ministration. The vancomycin levels in plasma were measured by HPLC and by fluorescence polarization immunoassay (FPIA) (TDX). The following correlati on was found: TDX=0.84 HPLC+1.04. The mean vancomycin levels in skin, fat, atrium, pericardium and sternum, before and after bypass, are reported. (C) 1998 Elsevier Science B.V. All rights reserved.