J. Navas-castillo et al., Improvement of the print-capture polymerase chain reaction procedure for efficient amplification of DNA virus genomes from plants and insect vectors, J VIROL MET, 75(2), 1998, pp. 195-198
A rapid and simple procedure is described to amplify efficiently geminiviru
s DNA genomes by improving the print-capture polymerase chain reaction (PCR
) procedure reported recently for RNA viruses. This method, termed print-PC
R (P-PCR), allows direct amplification of DNA from infected plant or whitef
ly tissues printed directly on Whatman 3MM paper, without the need of any g
rinding, incubation, or washing steps previous to the amplification reactio
n. P-PCR reduces sample manipulation and avoids previous extraction of nucl
eic acids, thereby diminishing the possibilities of cross-contamination bet
ween samples. P-PCR has been successfully applied to whiteflies and various
plant species infected by two different tomato yellow leaf curl viruses, T
YLCV-Sr and TYLCV-Is, and for the amplification of the full-length genome o
f TYLCV-Is from infected plants. (C) 1998 Published by Elsevier Science B.V
. All rights reserved.