Feline calicivirus capsid protein expression and capsid assembly in cultured feline cells

Authors
Geissler, K Schneider, K Fleuchaus, A Parrish, CR Sutter, G Truyen, U
Citation
K. Geissler et al., Feline calicivirus capsid protein expression and capsid assembly in cultured feline cells, J VIROLOGY, 73(1), 1999, pp. 834-838
Citations number
22
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
73
Issue
1
Year of publication
1999
Pages
834 - 838
Database
ISI
SICI code
0022-538X(199901)73:1<834:FCCPEA>2.0.ZU;2-K
Abstract
The capsid protein of feline calicivirus (PCV) was expressed by using plasm ids containing cytomegalovirus, simian virus 40, or T7 promoters. The stron gest expression was achieved with the T7 promoter and coinfection with vacc inia virus expressing the T7 RNA polymerase (MVA/T7pol), The FCV precursor capsid protein was processed to the mature-size protein, and these proteins were assembled in to virus-like particles.