M. Mathy-hartert et al., Protective activity of propofol, Diprivan (R) and intralipid against active oxygen species, MEDIAT INFL, 7(5), 1998, pp. 327-333
WE separately studied the antioxidant properties of propofol (PPF), Dipriva
n(R) (the commercial form of PPF) and intralipid (IL) (the vehicle solution
of PPF in Diprivan(R)) on active oxygen species produced by phorbol myrist
ate acetate (10(-6) M)-stimulated human polymorphonuclear leukocytes (PMN:
5 x 10(5) cells/assay), human endothelial cells (5 x 10(5) cells/assay) or
cell-free systems (NaOCl or H2O2/peroxidase systems), using luminol (10(-4)
M)-enhanced chemiluminescence (CL). We also studied the protective effects
of Diprivan(R) on endothelial cells submitted to an oxidant stress induced
by H2O2/MPO system: cytotoxicity was assessed by the release of preincorpo
rated Cr-51. Propofol inhibited the CL produced by stimulated PMN in a dose
dependent manner (until 5 x 10(-5) M, a clinically relevant concentration)
, while Diprivan(R) and IL were not dose-dependent inhibitors. The CL produ
ced by endothelial cells was dose-dependently inhibited by Diprivan(R) and
PPF, and weakly by IL (not dose-dependent). In cell free systems, dose-depe
ndent inhibitions were obtained for the three products with a lower effect
for IL. Diprivan(R) efficaciously protected endothelial cells submitted to
an oxidant stress, while IL was ineffective. By HPLC, we demonstrated that
PPF was not incorporated into the cells. The drug thus acted by scavenging
the active oxygen species released in the extracellular medium. IL acted in
the same manner, but was a less powerful antioxidant.